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KMID : 0545120050150020354
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.354 ~ p.361
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Molecular Cloning and Characterization of Alkaliphilic Phospholipase B (VFP58) from Vibrio fluvialis
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Ahn SH
Jeong SH/Kim JM/Kim YO/Lee SJ/Kong IS
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Abstract
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Vibrio fluvialis an enteropathogenic bacterium produces a phospholipase which is thought to be an important factor in the pathogenesis of disease. In this study the phospholipase gene (vfp) was identified from V. fluvialis (KCTC 2473) and its sequence was determined. The entire open reading frame was composed of 1689 nucleotides and 563 amino acids. The phospholipase gene (vfp) was overexpressed in Escherichia coli as a his-tag fused protein. This recombinant protein (rVFP58) was solubilized with 6 M urea and purified by Ni-NTA affinity chromatography. The action mode of rVFP58 was determined by TLC and GC-MS and it showed phospholipase B activity which had both phospholipase A and lysophospholipase activities. The rVFP58 showed a maximum activity at pH around 9- 10 and temperature of about 40oC and it was stable under alkaline condition over pH 9. The cytotoxicity of rVFP58 was evaluated using a fish cell line CHSE-214 and was found to cause significant cell death after 14 h of exposure to 250 μg of the protein.
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